Preparation of P. triticina inoculum
The causal fungus of leaf rust, P.triticina was isolated from in- fectedwheatleavescollectedfromthecommercialwheatfields and the Egyptian Wheat Rust Trap Nurseries during winter growing seasons of 2012/13 and 2013/14. Since the causal fungus of leaf rust is an obligate parasite, rust isolates were maintained on living plants. For multipli- cation of P. triticina uredospores, the collected samples were transferred onto seedlings of the highly susceptible wheat variety “Morocco” to obtain enough inoculum for further investigations.