Yeast strains were grown on agar plates containing
the proper media. For example, the untransformed Saccharomyces strains
(such as strain 1400 or AH22) were grown on YEPD, and the transformed
recombinant Saccharomyces strains [such as 1400(pLNH32)] were grown on
YEPX (1% yeast extract, 2% peptone, 2% xylose). To prepare the seed culture
for a new strain, first yeast cells from the agar plates were inoculated directly into
test tubes, each containing 5 ml of medium [YEPD for strain 1400 or AH 22 and
YEPX for 1400(pLNH32) and AH22(pLNH32)