Fig. 4. Vaccine trial II. Survival rates of fish vaccinated with different doses of live
VHSV following injection of Poly(I:C) or formalin-treated (FT) VHSV. (A) Fish were
intraperitoneally injected with VHSV (106
, 105
, and 104 TCID50 100 mL1 fish1
) 2 d after
injection of Poly(I:C) at a dose of 100 mg 100 mL1 fish1 or were intraperitoneally
injected with FT VHSV (106
, 105
, and 104 TCID50 100 mL1 fish1
) 2 d after injection with
DEPC-treated water. Control fish were injected with VHSV at doses of 106
, 105
, and 104
TCID50 100 mL1 fish1
. (B) Survivors from the groups of vaccinated fish were challenged
with VHSV at a dose of 106 TCID50 100 mL1 fish1
. Fish from the naïve group
were also challenged in the same manner.