In heterotrophic biofloc-based shrimp culture systems, the driving force is dense populations of active heterotrophic bacteria which can be promoted by increasing the C/N ratio of feed input and assimilate the wastenitrogen fromculturewater resulting in the production of newmicrobialbiomass (cellular proteins) (Avnimelech, 2006; Crab et al., 2007;Ebeling et al., 2006).