To Prepare MBL Medium Add 1mL of each stock solution (1 – 11) to 1 litre distilled water. (For species which cannot use nitrate substitute 1mL of NH4Cl made up to 5.4 g /L H2O) Adjust pH to 7.2 with HCl. Autoclave at 121°C (15PSI for 15 mins).
To Prepare MBL MediumAdd 1mL of each stock solution (1 – 11) to 1 litre distilled water.(For species which cannot use nitrate substitute 1mL of NH4Cl made up to 5.4 g /L H2O)Adjust pH to 7.2 with HCl.Autoclave at 121°C (15PSI for 15 mins).