In all studies, three replicates of 10 bed bugs were exposed to
each treatment by placing them on pre-sprayed, dry substrate in
a Petri dish for 1 h. After exposure, bed bugs were placed on clean
filter paper in a Petri dish. All bed bugs were fed on day 7, 14, and
21 following exposure and mortality recorded daily for 21 d.
Cadavers were incubated under high humidity to confirm mycosis.
Survival data were analyzed using Kaplan–Meier survival analysis
(SPSS, software version 18). Differences in median survival time
between treatments were compared using the log-rank test.
In all studies, three replicates of 10 bed bugs were exposed toeach treatment by placing them on pre-sprayed, dry substrate ina Petri dish for 1 h. After exposure, bed bugs were placed on cleanfilter paper in a Petri dish. All bed bugs were fed on day 7, 14, and21 following exposure and mortality recorded daily for 21 d.Cadavers were incubated under high humidity to confirm mycosis.Survival data were analyzed using Kaplan–Meier survival analysis(SPSS, software version 18). Differences in median survival timebetween treatments were compared using the log-rank test.
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