. In similar experiments, with extracts of horse chestnut and acorn, coagulation activity was the best at pH 3–4 and 8–10 and lower at neutral pH. The most appropriate pH for these extracts to perform coagulation, was pH 10 and they showed very low coagulation activity in range from pH 5 to 8 (Sˇciban ´ et al., 2009a).