Materials and Methods The green mature 65-70 days old capsules of Erythrodes humilis were collected from Sylhet, Bangladesh and washed under tap water to remove dust particles. After that, added few drops of teepol solution for few min and washed under running tap water for 10 min. The capsule was surface sterilized by immersing 0.1 % (w/v) HgCl for 10 min, 70% (v/v) ethanol for 30 sec and finally by washed three times with sterile distilled wate