For light microscopy, larvae were slide-mounted in Hoyer’s
medium. Cavity slides were used for first and second instars
and for the cephaloskeleton of third instars. Larvae were
photographed using a Nikon 8400 digital camera mounted on a
Nikon Eclipse E200 microscope (Nikon Corp., Tokyo, Japan).
Material prepared for SEM was dehydrated through 80.0%,