In Japan, fertilised chicken eggs are used to produce influenza vaccines; however, some
eggs die and then contamin ate the vaccine stock solution, which causes large economic
losses and raises health concerns. Therefore, a non -destructive test that allows distinction
of normal and unsuitable chicken eggs was develo ped, first using visible and near-infrared
(VNIR) spectros copy and subsequently a light-emit ting diode (LED) light source . Eggs were
sampled from an egg farm, and eac h of the eggs categorised as either normal or unsu itable
using candli ng. Linear discriminant analysis was applied using wavelength absorbance
data to distinguish between normal and unsu itable eggs. All of the optica l absorbance
values of the normal eggs were found to be higher than those of unsuitable eggs. To reduce
the pro duction cost, LED lights and photod iodes were used as the ligh t source and light
receptors, res pectively. A discrimi nation rate of 92.9% was obtai ned when LED was used as
the light source .