2.3. Synthesis of silver nanoparticles
For the synthesis of the silver nanoparticles, a certain volume
of the olive leaf extract (0.2–9) ml was added to the AgNO3
solution and the volume was adjusted to 10 ml with de-ionized
water. The final concentration of Ag+ was 1 · 103 M. The
solution was stirred for 2 min. The reduction process Ag+ to
Ag0 nanoparticles was followed by the color change of the
solution from yellow to brownish-yellow to deep brown
depending on parameters studied such as the extract concen-tration, temperature and pH. The nanoparticles were prepared
at different pH values, the pH of the solutions was adjusted
using 0.1 N H3PO4 or 0.1 N NaOH solutions.