The liquid samples from hydrolysis and SSF were analyzed by high performance liquid chromatography (HPLC) equipped with UV/vis and RI detectors (Jasco International Co., Tokyo, Japan).Ethanol and glycerol concentrations in the SSF samples were detected on an Aminex HPX-87H column (Bio-Rad, Richmond, CA,USA) at 60◦C with 0.6 ml/min eluent of 5 mM sulfuric acid.