Method Extraction Clarification
1 10 g sample homogenized in 15 mL hot water (50–60°), placed in a water bath 15 min Filter through filter paper and membrane filter
2 10 g sample homogenized in 10 mL hot water (50–60°), placed in a water bath 15 min 5 mL Carrez I and Carrez II. Filter through filter paper and membrane filter
3 10 g sample homogenized in 10 mL hot water (50–60°), placed in a water bath 15 min 5 mL of acetonitrile. Filter through filter paper and membrane filter
4 10 g sample homogenized and dried in an oven + 1 g activated charcoal in 20 mL hot water (50–60°), placed in a water bath 15 min 5 mL of acetonitrile. Filter through filter paper and membrane filter
5 10 g sample homogenized in 10 mL hot water (50–60°), placed in a water bath 15 min 5 mL of acetonitrile. purified with sep-pak C18 (2 mL methanol and 4 mL water, load 1 mL sample, wash with 3 mL × 1 mL water, elute with 2 mL 0.5 M NaCl solution
Method Extraction Clarification1 10 g sample homogenized in 15 mL hot water (50–60°), placed in a water bath 15 min Filter through filter paper and membrane filter2 10 g sample homogenized in 10 mL hot water (50–60°), placed in a water bath 15 min 5 mL Carrez I and Carrez II. Filter through filter paper and membrane filter3 10 g sample homogenized in 10 mL hot water (50–60°), placed in a water bath 15 min 5 mL of acetonitrile. Filter through filter paper and membrane filter4 10 g sample homogenized and dried in an oven + 1 g activated charcoal in 20 mL hot water (50–60°), placed in a water bath 15 min 5 mL of acetonitrile. Filter through filter paper and membrane filter5 10 g sample homogenized in 10 mL hot water (50–60°), placed in a water bath 15 min 5 mL of acetonitrile. purified with sep-pak C18 (2 mL methanol and 4 mL water, load 1 mL sample, wash with 3 mL × 1 mL water, elute with 2 mL 0.5 M NaCl solution
การแปล กรุณารอสักครู่..