3.7. Extracts/fractions and reference antioxidants
Three different standards known for their good antioxidant activity, trolox, ascorbic acid and quercetin were used. These standards were suggested by Parejo et al. (2000) who used them to evaluate scavenging activity by luminol chemiluminescence and DPPH assays. The radical scavenging activity of the standards increased in the following order: Ascorbic acid < Trolox < Quercetin.
Trolox was found to be 7.2 times more active than ethyl acetate fraction, ascorbic acid was 9.1 times more active and quercetin 10.9 times more active. Further, trolox was found to be 9.9 times more active than the ether fraction, ascorbic acid 12.5 times and quercetin 15 times. Another relatively active fraction was the butanol fraction. Trolox was found to be 15.8 times more active than butanol fraction, ascorbic acid 19.8 times more active and quercetin 23.7.
3.7. Extracts/fractions and reference antioxidantsThree different standards known for their good antioxidant activity, trolox, ascorbic acid and quercetin were used. These standards were suggested by Parejo et al. (2000) who used them to evaluate scavenging activity by luminol chemiluminescence and DPPH assays. The radical scavenging activity of the standards increased in the following order: Ascorbic acid < Trolox < Quercetin.Trolox was found to be 7.2 times more active than ethyl acetate fraction, ascorbic acid was 9.1 times more active and quercetin 10.9 times more active. Further, trolox was found to be 9.9 times more active than the ether fraction, ascorbic acid 12.5 times and quercetin 15 times. Another relatively active fraction was the butanol fraction. Trolox was found to be 15.8 times more active than butanol fraction, ascorbic acid 19.8 times more active and quercetin 23.7.
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