Determination of total phenolics content
The total phenolics content in investigated extracts of buckwheat and
wheat flours, measured as gallic acid equivalents, were determined spectrophotometrically
using Folin-Ciocalteau's reagent (S i n g l e t o n et al., 1999). The
extract (0.1 mL) of buckwheat or wheat flours was diluted with distilled water
(7.9 mL). Folin-Ciocalteau's reagent (0.5 mL) and sodium carbonate solution
(1.5 mL; concentration 20 g/100 mL) were added, and the reaction mixture
was mixed thoroughly. The mixture was allowed to stand for 120 min with intermittent
shaking. The absorbance was determined in a spectrophotometer
Jenway (6405 UV/Vis) at 750 nm.