When
the distilled water went to the detection zone, the time of test
paper in running buffer was nearly 1 min and the red color of different
concentrations of glucose solution can be judged by eyes
semiquantitatively or analyzed the pictures taken by camera or
cell phone with Quantity One software. In our experimental process,
camera and parameters are as follows: Canon 600D digital
camera with CMOS sensor (Taiwan, China), maximum resolution
5184
×
3456 pixels, programmed auto exposure without flash light,
the object distance is 30–40 cm. These photos were imported into
the computer and color differences were analyzed by image software
Quantity One (Bio-Rad company). Darkness density of each
dot was picked to describe color difference among the volume data
in Volume Analysis Report. The calibration curves were obtained
by spotting different concentrations of standard glucose solutions
to the substrate zones.