Natural rubber (cis-1,4-polyisoprene) is a natural polymer that is extracted from rubber tree in the form of latex. It is a colloidal system composed of hydrocarbon, protein and phospholipid. Natural rubber latex usually has dry rubber content (DRC) about 30-33% before it is centrifuged to yield concentrated latex and skim latex having DRC of 60% and 4-6%, respectively. Concentrated latex is normally preserved with 0.7%wt ammonia and it is called high ammonia natural rubber latex (HANRL). Natural rubber latex is a raw material for the production of many products such as gloves, condoms and balloons. However, the applicability of products from natural rubber latex is in some degree limited because some people are allergic to proteins in the rubber. Regarding that aspect, there are many methods to reduce the protein content such as leaching, chlorination, depolymerization of proteins using proteolytic enzymes, and denaturation using nonionic surfactant (Abhilash et al., 2009). In this work, we focus on denaturation method in which rubber particles are deproteinized with urea and SDS in continuous flow process about which only a few works have been done to investigate this application.