A UV spectrum for the solution (10 µg mL–1) of Isotretinoin
was recorded in a 10 mm cell over the range
200-400 nm using methanol in the reference cell. Isotretinoin
showed maximum absorbance at 340 nm (Figure
2) while Erythromycin was detected at 410 nm after
derivatization with 10% H2SO4 and heating at 100 °C for
15 min