A replicationdefective
adenoviral vector containing the mIL-12 cDNA
under the transcriptional control of the Rous sarcoma virus
long terminal repeat promoter (ADV/mIL-12) was constructed
and plaque purified as followed. The cDNA from
both mIL-12 subunits were cloned by RT-PCR from total
RNA obtained from pockweed mitogen stimulated mouse
splenocytes.