Identification and quantification of the oligosaccharides was
achieved by ion exchange chromatography with a pulsed amperometric
detector (HPLC-PAD). Chromatographywas performed
on a Carbopac PA-100 column at 22–24 ◦C, using a GP50 gradient
pump, ED40 electrochemical detector and the software
PEAKNET, all from DIONEX (USA). The sugars were eluted
in 50mM sodium hydroxide with a linear gradient of sodium
acetate (0–200 mM), at a flow rate of 1ml min−1.