3.4. Methanol Extract of Triphala Induced Apoptosis in a p53 Independent Manner
The ability of MET to induce apoptosis in HCCSCs and cells with shRNA-attenuated p53 was measured by PARP cleavage, the hallmark of apoptosis, using western blotting. Figure 4 shows the induction of PARP cleavage as accumulation of cleavage fragment (89 kDa) in MET treated cells. Triphala extract (MET) induced apoptosis in HCCSCs independent of p53 was supported by increased PARP cleavage (Figures 4(a) and 4(b)).