The sample volume need not be 1.00 ml as long as the capacity of the linear range is not exceeded and conversion calculations and standards reflect the change. Microadaptation reduces costs. This procedure has been scaled down by a factor of five to a final volume of 20.0 ml.9'11For that procedure, 2.00 ml of a 1:10 diluted sample (compared to the 100-ml procedure), 10.0ml of FCR diluted 1:10, and 8.00 ml of 75 g/liter sodium carbonate gave the final 20.0 ml. Use of semiautomatic manual pipettes and syringe diluters and dispensers at the same final volume (20 ml) was also satisfactory with only a very slightly increased standard deviation. With adequate equipment, further size reduction is certainly possible.