The headspace of a BCE03 is significantly larger compared to the BC25 (see illustration below). Referencing previous lab reports, holdup volume for a BCE03 capsule is ~1.3 L pre-blowdown, and ~180 mL post-blowdown, while that for a BC25 is only ~12 mL. Without conducting a post-use blowdown, target protein may also be lost together with the residual product.