Six sterile porcelain cylinders with outside diameter of 8 mm and length 10 mm were placed on the base layer about 608 Fifty microliters each of reference standard (10 mM amphotericin) and test compound were added in each well. Three alternate wells on each plate were filled with a reference standard and the remaining three were filled with test solution. The reference standard was used for the verification of strain sensitivity/resistance pattern. Inhibition area was measured after overnight incubation at 30 C. Screening for antifungal activity