Carbonation procedure
The system for injecting carbon dioxide in the beverage for
the study was developed by modifying a domestic carbonator
. The carbonator was fitted with
a 1 kg CO2 gas cylinder along with necessary attachments of
regulators and pressure gauges. The system was leak- tested
before each trial using the standard soap bubble test. The glass
bottle containing FDD was placed on the system platform
such that the gas injection pipe remained dipped in the sample
during carbonation. The bottle was raised using the platform
lever of the carbonator to snugly fit to the top seal ring. The
pressure of the gas in the injection line was manually adjusted
to required levels and the gas was then flushed into the
beverage by pressing the injection button for the required
time. At the end of carbonation, the bottle platform was
lowered; the bottle collected and immediately capped with
gas leak proof screw caps.