Fig. 4. CGE1 expression under cold stress temperature. (A) Quantification of theCGE1a and CGE1b transcript levels by real-time PCR. Relative expression levels werenormalized with the gene expression of RACK1. Accumulation of the CGE1a andCGE1b transcripts during heat shock at 40◦C is given for a comparison (insert). (B)Analysis of CGE1 levels by protein gel blot using CGE1-specific antibodies. Totalprotein corresponding to 2 g chlorophyll was extracted from samples taken fromcultures at the time points indicated. -CrPII signal served as a loading control.Significant differences at the 95% level with respect to the control are indicatedwith **. Cells were treated as described in the legend to Fig. 2A.