Cotton textile and clothing industry is a complex and multi-tiered system that consists of cotton culti-vation and harvesting, fiber production, yarn manufacturing, fabric preparation, fabric processing thatincludes bleaching and dying sub-processes among others and fabrication of the final product. An array ofenvironmental concerns are associated with this sector, the most significant of which are issues relatedto use of agrochemicals in the cultivation of cotton and water, energy and chemical consumption in thefabric processing stage. Textile industry is a significant contributor to the Turkish economy constituting18% of total export volume in 2013 according to Turkish Statistical Institute. In the study, environmen-tal impacts of Eco T-shirts produced from organically grown cotton and processed with green dyeingrecipe were compared to that of conventional T-shirts, in terms of their contributions to global warming,acidification, aquatic and terrestrial eutrophication and photochemical ozone formation using life cycleassessment methodology. The results reveal that Eco T-shirts have lower impact potentials across allinspected categories, with the most dramatic reduction in aquatic eutrophication potential (up to 97%)due to elimination of nitrogen and phosphorus containing chemical based fertilizers. The results alsoshow that global warming potential is by far the largest environmental impact for both conventional andEco T-shirts with the main impact coming from use phase, followed by cultivation and harvesting andfabric processing phases. The results of the analysis underline the importance of utilizing sustainable rawmaterials in all life cycle stages of cotton textile products and the necessity of focusing on the consumerbehavior and sustainable practices in the use phase of the products as well.