The present study investigated the effect of three different gum flavors and a No Gum Control on symptoms of negative affect associated with nicotine withdrawal in college-aged smokers. The three gum conditions (peppermint, vanilla, and baked apple cardamom) were used to allow for an evaluation of the differential effect of flavor. Peppermint was chosen because it is the most widely used gum flavor in the U.S. according to Wrigley Manufacturing Company, LLC. The other two sweet, non-commercially available flavors were chosen as it was believed that these flavors would be perceived by participants as relaxing. The primary research question was to assess if flavored chewing gum would help with symptoms of negative affect during an extended (48 h) abstinence period. It was predicted that there would be lower levels of negative affect when participants were asked to chew gum compared to the no gum control condition. Secondary research questions examined the extent to which gum flavor enhanced or diminished this effect as well as the effect on overall nicotine withdrawal.