Effect of coconut water on shoot regeneration and shoot length
From table 1 it was observed that 50% explants differentiated into shoots when the coconut
water was not supplemented to the medium and produced 2.45 shoots per explant and in this
condition shoot length was 2.32 cm on an average. When the coconut water was added in themedium, a significant increase in the frequency of explants showing shoot regeneration was
observed and 75% of explants showed shoot regeneration in the presence of 100 mL L-1
coconut water followed by 74% explants in 150 mL L-1 coconut water in the medium. Use of
coconut water significantly affected multiple shoot regeneration from the explants. Whereas
4.2 shoots per explant were observed in the medium containing 100 mL L-1 coconut water
followed by 3.84 shoots per explant in medium containing 150 mL L-1 coconut water.
Developed shoots showed significant increase in length as the concentration of coconut water
was increased up to 100 mL L-1. Highest shoot length i.e. 4.52 cm was observed in the
medium containing 100mL L-1 coconut water. Increasing the concentration of coconut water
in the medium above 100 mL L-1 did not showed any significant effect on shoot regeneration.