To include cell bioreactors in a LOV manifold, a loop
connects Port 3 of the LOV and the flow cell, as shown in
Fig. 5A, thus allowing an aliquot of beads to be delivered
through that port and captured in a microcolumn behind
the bead-retention plug–nozzle directly upstream
from the flow cell. Once the microcolumn is packed, it
can be perfused by the auxiliary syringe pump through
the loop with the resulting perfusate entering the flow
cell through the plug–nozzle. Inside the flow cell, the
perfusate is mixed with reagent for analyte detection
[36]. This manifold can be modified by connecting a
multiposition valve (MPV) to Port 2 and deleting the
loop connected to Port 3
As shown in Fig. 4A, one of them uses the detection
cell of the LOV manifold as interface, and a two-way
isolation valve placed in the outlet port of the detection
cell for hydrodynamic sample injection, refreshing the CE
buffer, or automated capillary flushing–reconditioning.
A bare platinum wire was used as the cathode placed in
a container filled with the CE buffer. The anode is housed
in a PEEK tube and connected away from the LOV
manifold. The capillary was enclosed in a PEEK tube and
the capillary window was adjusted to the light path of
the two optical fibers in the LOV manifold. CE separation
was driven by a high-voltage power supply, with the
cathode side away from the LOV manifold and the whole
system controlled by the personal computer that controls
the LOV system [24