5. Conclusions
In this study, strain T. trogii Berk S0301 was selected from 18 Trametes species for having the highest ABTS oxidization ability and a close correlation between ABTS oxidization and MG decolorization. The decolorization efficiency of MG by laccase of T. trogii Berk S0301 was only slightly affected by the presence of high concentrations of metal ions (Mn2+, Mg2+, Zn2+, Co2+ and Na+) and by temperatures above 50 °C. Decolorization of other dyes at 60 °C also demonstrated that T. trogii Berk S0301 laccase possessed good thermostability. Additionally, phytotoxicity and microbial toxicity assays revealed that less toxic metabolites of MG were formed after treatment with T. trogii Berk S0301 laccase. These results show T. trogii Berk S0301 and its laccase may facilitate novel and more efficient bio-catalytic process applications.