The aerial part ofPhyllanthus amarusSchum. et. Thonn. was
collected from the Faculty of Pharmaceutical Sciences, Chulalongkorn University during May–June, 2005. The plant was
identified by Assoc. Prof. Dr. Chaiyo Chaichantipyuth, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences,
Chulalongkorn University. Fresh aerial part was dried thoroughly under shade and cut into small pieces and added with
deionized water about 2 cm over, boiled for 15 min and filtered
through three layers of fine cleaned gauze. Residues was boiled
again in deionized water for 15 min and then filtered. The filtrate
of both extractions were combined and centrifuged at 2000 rpm
for 10 min. The supernatant was filtered using the filter paper
no. 1. The clear filtrate was lyophilized to dry by a freeze dryer
and kept in the protected light bottle at 4◦
C.
The yield of the aqueous extract (PA) was 17.42% (w/w) from
the starting dried aerial part. Phyllanthin and hypophyllanthin
were found in this PA aqueous extract using preliminary TLC
technique. A fresh preparation was prepared by dissolving in
The aerial part ofPhyllanthus amarusSchum. et. Thonn. was
collected from the Faculty of Pharmaceutical Sciences, Chulalongkorn University during May–June, 2005. The plant was
identified by Assoc. Prof. Dr. Chaiyo Chaichantipyuth, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences,
Chulalongkorn University. Fresh aerial part was dried thoroughly under shade and cut into small pieces and added with
deionized water about 2 cm over, boiled for 15 min and filtered
through three layers of fine cleaned gauze. Residues was boiled
again in deionized water for 15 min and then filtered. The filtrate
of both extractions were combined and centrifuged at 2000 rpm
for 10 min. The supernatant was filtered using the filter paper
no. 1. The clear filtrate was lyophilized to dry by a freeze dryer
and kept in the protected light bottle at 4◦
C.
The yield of the aqueous extract (PA) was 17.42% (w/w) from
the starting dried aerial part. Phyllanthin and hypophyllanthin
were found in this PA aqueous extract using preliminary TLC
technique. A fresh preparation was prepared by dissolving in
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