For pre-culture preparation, 30 mL of lychee juice were inoculated separately with 3 mL each of T. delbrueckii PRELUDE and S. cerevisiae MERIT.ferm, which were incubated at 30C and 25c, respectively for 48 h under static conditions and yeasts grew to over 10-7 CFU/mL. During the fermentation, another 15 mL of S. cerevisiae MERIT.ferm pre-culture were prepared as mentioned above for sequential fermentation.