The thin-layer chromatography (TLC) profile of G. applanatum extract. Crude
chloroform extract (CC) (5e10 ml) of G. applanatum yielded 5 spots with variable
fluorescing abilities; partial purified compound (PP) (2 ml) exhibited 2 spots; and fully
purified compound (FP) (2 ml) showed a single fluorescent spot under long UV range
(254e365 nm). The TLC plate was developed using gradient solvent system with
chloroform and petroleum ether (8:2) as mobile phase.