Then, the homogenated sample was serially diluted from 10-1 to 10-6. And, 0.1 ml of sample was
taken from 10-4 , 10-5, and 10-6 diluted sample, followed by spreading over them on NA and NB medium separately
for bacteria, and the PDA for fungi. One media from each of them was maintained as a control without sample. The
plates were incubated at 37oC for 24 to 48 hours and the bacterial colonies was calculated using TPC Method.