[Shaken until dissolved, then adjusted to pH 6.8 with HC1 (conc.). This recipe is
enough for nine samples and a blank. Fresh enzyme solutions should be prepared for
each day's testing.]
Each vial was then capped and shaken lightly to wet the hair clippings. A blank
sample, of just 10.0 g of enzyme solution, was also prepared. All of the samples were
then placed in a block heater or in an oven set at 63øC _+ 3 ø for three days, swirling
each sample at least once during the heating cycle to make sure that all of the hair
sample is in the solution and not adhering to the sides of the vial.
To each sample, seven milliliters of methyl isobutylketone (MIBK) and five hundred
microliters (0.5 ml) of concentrated reagent hydrochloric acid were added. The samples
were then placed in a Burrell mechanical shaker and agitated for 30 minutes. The
extraction vials were then spun in an IEC Clinical © centrifuge at moderate speed for
20-30 minutes to permit phase separation. The 4.0-dram vials will fit into 50-ml
centrifuge shields. Following separation, the top (solvent) layer of each sample is drawn
off with a transfer pipet and reserved in a new vial for AAS analysis.
[Shaken until dissolved, then adjusted to pH 6.8 with HC1 (conc.). This recipe is
enough for nine samples and a blank. Fresh enzyme solutions should be prepared for
each day's testing.]
Each vial was then capped and shaken lightly to wet the hair clippings. A blank
sample, of just 10.0 g of enzyme solution, was also prepared. All of the samples were
then placed in a block heater or in an oven set at 63øC _+ 3 ø for three days, swirling
each sample at least once during the heating cycle to make sure that all of the hair
sample is in the solution and not adhering to the sides of the vial.
To each sample, seven milliliters of methyl isobutylketone (MIBK) and five hundred
microliters (0.5 ml) of concentrated reagent hydrochloric acid were added. The samples
were then placed in a Burrell mechanical shaker and agitated for 30 minutes. The
extraction vials were then spun in an IEC Clinical © centrifuge at moderate speed for
20-30 minutes to permit phase separation. The 4.0-dram vials will fit into 50-ml
centrifuge shields. Following separation, the top (solvent) layer of each sample is drawn
off with a transfer pipet and reserved in a new vial for AAS analysis.
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