and Supporting Information Video). Interestingly, signals from
lysosomes did not colocalize with signals from the endosomes
that contained PNA
NFκb‑flu
/MPOC
(arrow 1, Figure 5 row
2), indicating that the endosomes filled with PNA
Cou
and
MPOC
were not merging with lysosomes. Noted that a
normal merging of lysosomes with the endosomes that
contained no PNA
Cou
NFκb‑flu
/MPOC
could still be observed
(arrow 2, Figure 5 row 2). Here the emission from the
lysosome specific dye started to fade at ≈150 min, and
throughout the 150 min observation, no merging of lysosome
and PNA
NFκb‑flu
/MPOC
Cou
Cou
-containing endosome was observed.
After 90 min incubation, obvious fluorescence signals from
PNA
NFκb‑flu
and MPOC
could be observed throughout the
cytoplasm and nucleus.
and Supporting Information Video). Interestingly, signals fromlysosomes did not colocalize with signals from the endosomesthat contained PNANFκb‑flu/MPOC(arrow 1, Figure 5 row2), indicating that the endosomes filled with PNACouandMPOCwere not merging with lysosomes. Noted that anormal merging of lysosomes with the endosomes thatcontained no PNACouNFκb‑flu/MPOCcould still be observed(arrow 2, Figure 5 row 2). Here the emission from thelysosome specific dye started to fade at ≈150 min, andthroughout the 150 min observation, no merging of lysosomeand PNANFκb‑flu/MPOCCouCou-containing endosome was observed.After 90 min incubation, obvious fluorescence signals fromPNANFκb‑fluand MPOCcould be observed throughout thecytoplasm and nucleus.
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