culture medium
based on Dulbecco’s modified Eagle’s medium and Ham’s
F-12 nutrient mixture (1:1) and containing 0.75% BSA
(essentially fatty acid free; Sigma–Aldrich, St. Louis, MO,
USA), 5% serum (2.5% Fetal Bovine Serum; Greiner Bio-One,
Frickenhausen, Germany; and 2.5% Newborn Calf Serum;