Abstract—Glucose production from cellulose flakes with cellulases was improved after pretreatment with saturated CaCl2 at room
temperature. When pretreated microcrystalline cellulose flakes (Funacel II, Funakoshi Co., Ltd, Tokyo, Japan) were saccharified
with the cellulases, 76.8% of the substrate was converted into glucose within 5 h, whereas the corresponding conversion rate of
water-pretreated cellulose flakes was 33.8%.