The cells were then layered over 2 ml of Lympholyte-M (Cedarlane Laboratories) and centrifuged at 1,300 g for 20 min at room temperature. Cells were removed from the interface, washed once, and counted.
The cells were then layered over 2 ml of Lympholyte-M (CedarlaneLaboratories) and centrifuged at 1,300 g for 20 min at room temperature. Cellswere removed from the interface, washed once, and counted.