Screening for antifungal activity. Endophytic fungi were screened by dual culture method for antifungal activity against potentially pathogenic fungi of chili pepper, Phytophthora capsici, Colletotrichum acutatum and Fusarium oxysporum.
The phytopathogenic fungi were collected from the culture stock of Plant Pathology Laboratory, Chungnam National University, Daejeon, Republic of Korea. Five-dayold
disks (5 mm diameter) of endophytes were placed on 3 points of Petri plates containing PDA medium.
Test pathogens were inoculated at the center of PDA plates.
Plates were incubated at 25oC for 5−8 days.
Antifungal activity was assessed by the size (diameter in mm) of the
inhibition zones.
Each experiment was repeated three times