To test whether the curcumin-induced loss of virus infectivity is irreversible, 50 pfu of PR8 viruses
were incubated with 30 mM of curcumin or DMSO in a total volume of 500 ml infectious medium. One hour after incubation, inoculums were injected
into the allantoic sac of 4 embryonated hen’s eggs. Treated eggs were incubated in 37uC incubator for 18 hours, the yield of virus progeny was
determined by HA test