Various treatments of imperforate hymen have been described, including fenestration [5]; ovariohysterectomy with removal of the cervix and cranial half of the vagina [3, 4]; and, if vestibulovaginal stenosis is present, resection of the stenotic region followed by vestibulovaginal anastomosis through an episiotomy [10]. All of these techniques have resulted in resolution of clinical signs. However, in one instance of imperforate hymen with secondary pyocolpos that was treated by endoscopic removal of the membrane, the dog developed peritonitis and sepsis after the procedure and died [6]. In cases of infection, it may be more prudent to either resect the infected tissue or at least place drains. In humans, imperforate hymen is treated surgically by making a cruciate incision over the hymen and excising the redundant membrane [11]. Special care should be taken during this process to avoid tearing the vaginal wall. Because of the severe clinical signs associated with hydrocolpos secondary to imperforate hymen, use of a technique that either ablates the tissue or establishes permanent vaginal outflow patency is highly recommended to prevent recurrence of clinical signs. In the presented case, digital perforation and endoscopic removal of membrane remnants were successful in establishing patency and resolving clinical signs.