Meat cut from different anatomical parts of adult cattle were
ground, mixed and divided into 4 batches of 50 kg each. The first
batch, representing lot 1,was formed into patties, directly packaged
and used as control. The other batches were inoculated with a
mixture of LAB and coagulase-negative, catalase-positive cocci
(CNCPC) at a final concentration of 105 CFU/g of product before
being formed into patties. Lot 2 was inoculated with a mix of
Lactobacillus sakei subsp. carnosus/L. sakei þ Staphylococcus xylosus
at a ratio of 1/1. Lot 3 was inoculated with Lactococcus lactis ssp.
lactis/L. sakei þ S. xylosus at a ratio of 1/1. Lot 4 was inoculated with
L. sakei subsp. carnosus/L. sakei þ S. xylosus at a ratio of 1/2. The
burgers were packed in MAP, consisting of 70% O2 and 30% CO2, and
placed inside 15 10 3 cm rectangular trays of 200 mm in
thickness made of PET/PE/EVOH/PE ANTIFOG e EVOH. The trays
were laminated with a top film consisting of APET/PE/EVOH/PE. The
packaged burgers were stored at 4 ± 2 C for 12 days in artificial
light. At 0, 6, 9 and 12 days, 10 boxes were collected for microbiological,
and physical-chemical analyses. All analyses were conducted
in duplicate on three replicates at each sampling point.