The human gastric adenocarcinoma cell line AGS was
obtained from the Food Industry Research and Development
Institute (Hsinchu, Taiwan) and was maintained in
Ham’s F-12 medium (GIBCO BRL, Grand Island, NY, USA)
with 10% fetal calf serum (FCS). The other human gastric
cancer cell line, MKN45, was obtained from the Health
Science Research Resources Bank in Japan and was maintained
in RPMI 1640 medium (GIBCO BRL) containing 10%
FCS. The cells were subcultured every 2 days.