2.4. Determination of NIH 3T3 Cell Viability via Trypan Blue Cell Count Assay
Trypan blue cell count was carried out to identify the amount of viable cells present in each sample. After 24 h incubation period, harvested cell suspension (10 μL) was added with equal volume of 0.4% trypan blue stain. Hemocytometer was used for cell counting under inverted light microscope (NIKON, Japan). Viable cells are those excluded from the stain.