2.2.3. Test Procedure of AFB1
According to Ridascreen Aflatoxin B1 30/15 (Art no.: 1211) test kit manual [24], 50 μL of the standard solutions or prepared sample in duplicate was added to the wells of microtiter plate. Then 50 μL of the enzyme conjugate and 50 μL of the anti-aflatoxin antibody solution were added to each well, mixed gently and incubated for 30 min at room temperature (20–25°C)