Promoters, which are located upstream of structural genes and have a binding site for RNA
polymerase, regulate gene transcription by recognizing and binding to transcription factors. The
processes involved in regulating eukaryotic gene expression are complex, and promoter-mediated
control at the transcription level is an important aspect of the overall mechanism. Elucidating
how promoters regulate transcription requires information from many sources, for example,
cloning, confirmation of promoter function, and analysis of the interactions between promoter and
transcription factor