Ninety-two adult patients, 46–60 years old, with gas -
trointestinal cancer T3-4N0-xM0-y stages were included in
this study. Sixty-eight non-cancerous persons, 25–70
years old without gastrointestinal tract pathology formed
a control group and were also examined. The study protocol
was approved by Ethical Committee permission of M.
Gorky Donetsk National Medical University for research
involving humans. The features of enzymes activity were
studied in blood serum and lymphocytes. TK activity was
determined by the radioisotope method as described
previously [4]. It was given in nanomoles of thymidilate
formed within an hour per 1 mg of protein. TP activity
was determined spectrophotometrically [4] at 300 nm
according to the amount of thymine formed. 5’-NT
activity was determined by the number of inorganic
phosphate, ADA – at 265 nm according to the amount of
inosine formed [4]. Protein concentration in biological
material was determined using the method described by
Lowry et al. [5]. Statistical analysis of results was
performed using Medstat software package. Significance
of differences was analyzed by parametric and non parametric
methods.