Know the parts of a microscope.
2) What is working distance? What is parfocal distance?
3) What is magnification? What is resolution?
4) How do you perform a Gram stain? What color does this stain bacteria?
5) What microorganisms are eukaryotes? What microorganisms are prokaryotes?
6) What is the difference between autotrophic and heterotrophic bacteria?
7) What do you call the type of bacteria that can grow at cold temperatures (<10 oC)? At hot temperatures (80 oC)? At temperatures close to that of the human body?
8) What is the type of bacteria that grows at high salt concentration called?
9) What is the type of microorganisms that grows with little water called?
10) What is the type of bacteria that that can live at high temperature for a short time called?
11) What does the term, nutrient, mean? What is the difference between a macronutrient, micronutrient and a trace element? Give 2 examples of each.
12) Lead, Cadmium and Arsenic are very toxic to humans. Are their microorganisms that can use these elements?
13) What is the difference between a derived culture medium, a complex culture medium, and a differential culture medium?
14) What is Agar? How is Agar sterilized?
15) Describe 3 aseptic techniques.
16) How is the streak plate technique done?
17) What is meant by the term, free energy of a chemical reaction? What does an enzyme do to free energy of a chemical reaction?
18) What is meant by the term, enzyme inhibition?
19) Phases of microbial growth
20) Doubling times
21) Equation for exponential growth
22) Turbidometric measurements of cell numbers
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